• 货号：AS09 501a
    抗体名：兔抗拟南芥过氧化氢酶多克隆抗体
    抗体英文名：Cat|Catalase (peroxisomal marker)
    浓度：见说明书
    应用范围：western blot (WB)
    宿主：兔
    适应物种：植物
    标记物：无
    克隆性：否
    保存条件：低温
    亚型：IgG
    规格：200 µl

    供应商：上海经科化学科技有限公司 

     

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    Cat|Catalase (peroxisomal marker)介绍：

    货   号：AS09 501

    中文名称：兔抗拟南芥过氧化氢酶多克隆抗体

    英文名称：Cat|Catalase (peroxisomal marker)

    应用：western blot (WB)

    规格：200 µl

    价格：3975元

     

    Cat|Catalase (peroxisomal marker)简介：

    PRODUCT INFORMATION
    background 

    Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.
    immunogen 

    KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thalianaisoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547,At1g20620);
    host 

    rabbit
    clonality 

    polyclonal,
    purity 

    serum
    format lyophilized
    quantity 50 µl
    storage 

    store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    tested applications 

    western blot (WB)
    related products 

    AS08 374 | anti-KatG | catalase peroxidase (HPI), cyanobacterialAS09 501PRE | anti-Cat | Catalase (peroxisomal marker)
    additional information This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase.
    APPLICATION INFORMATION
    recommended dilution  

    1: 1000 with standard ECL (WB)

    expected | apparent MW  

    57 | 55  kDa

    confirmed reactivity  

    Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum, Spinacia oleracea, Zea mays, Vitis vinifera

    predicted reactivity  

    Triticum aestivum, Betula pendula, Citrus sp., Citrus maxima, Citrus clementina, Camellia sinensis, Litchi chinensis, Hibiscus cannabinus, Saccharum officinarum, Cucurbita maxima, Cucurbita moschata, Morus notabilis, Brassica napus, Brassica rapa subsp. pekinensis, Brassica campestris, Prunus persica, Fragaria ananassa, Brachypodium distachyon, Raphanus sativus, Sesamum indicum Paenibacillus sp.        dicots including: Cucumis sativus, Gossypium mexicanum, Glycine max, trees: Populus jackii, Pinus pinea, Elaeis guineensis var. tenera, Avicennia marina, Eucalyptus grandis.  

    not reactive in  

    Chlamydomonas reinhardtii

    additional information  

    To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.

    selected references   Kataya et al. (2015). Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation. Plant Physiol. 2015 Feb;167(2):493-506. doi: 10.1104/pp.114.254409.  Pilati et al. (2014). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14(1):87.  Blume et al. (2013).A possible role for the chloroplast pyruvate dehydrogenase complex in plant glycolate and glyoxylate metabolism. Phytochemistry Aug2.   Li et al. (2013). LESION SIMULATING DISEASE1 Interacts with Catalases to Regulate Hypersensitive Cell Death in Arabidopsis. Plant Physiol. Aug 19. Marok et al. (2013).  A drought-sensitive barley variety displays oxidative stress and strongly increased contents in low-molecular weight antioxidant compounds during water deficit compared to a tolerant variety. J. Plant Physioology, Feb 8.  

     

    \"western

    10 µg of total protein from Arabidopsis thaliana Col0 (1), Cat2-(Col0) (2), Ler0 (3), Cat2-(Ler0) (4), Zea mays (5), Oryza sativa (6),Brassica oleracea (7), Nicotiana bentamina (8) were extracted with 60mM Tris pH 6.9, 10mM DTT, 20% glycerol, 1mm PMSF were separated on 12.5% SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 3% skim milk in PBS+0.05% Tween20 for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in the same buffer. The antibody solution was decanted and the blot was rinsed briefly three times, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:50 000 in 3% skim milk in PBS+0.05% Tween20 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Western Lightning Plus-ECL ( PerkinElmer )according to the manufacturers instructions. Exposure time was  5min. in ChemiDoc XRS+ (Biorad ).